Junction Entry Lane Step

Gallery / Merge & Convergence

A section already carrying the line above cannot also hand the line below it the lane its feeder junction arrives on, so no lane assignment levels the run. The connector draws the 4px difference as a step -- flat, a 45-degree diagonal, flat -- instead of smearing it across the whole gap as a shallow slant (issue #1816).

Mermaid source

examples/topologies/junction_entry_lane_step.mmd

%%metro title: Junction entry lane step
%%metro line: riboseq | Ribo-seq | #e6007e
%%metro line: rnaseq | Matched RNA-seq | #2db572
%%metro line: tiseq | TI-seq | #2b6cb0
%%metro line: annotation | Hybrid annotation | #f2b407

graph LR
    subgraph preprocessing [Read pre-processing]
        infer_strand -->|riboseq,rnaseq,tiseq| equalise
    end
    subgraph alignment [Alignment & quantification]
        umi_dedup[UMI-tools dedup]
        salmon_quant -->|riboseq,rnaseq,tiseq| counts_out
    end
    subgraph novel_transcripts [Transcript discovery]
        stringtie -->|rnaseq| hybrid_merge
    end
    subgraph orf_calling [ORF discovery & calling]
        star_hybrid -->|riboseq| ribocode
        ribotish -->|riboseq| orf_merge
    end
    subgraph te [Translational efficiency]
        te_prep_orf -->|rnaseq| anota2seq
        anota2seq -->|riboseq| multiqc_final
    end
    equalise -->|riboseq| star_hybrid
    umi_dedup -->|rnaseq| stringtie
    umi_dedup -->|riboseq| ribotish
    salmon_quant -->|rnaseq| te_prep_orf
    hybrid_merge -->|annotation| star_hybrid

CLI command

nf-metro render examples/topologies/junction_entry_lane_step.mmd -o junction_entry_lane_step.svg

Rendered map