Bt Perp Left Entry Right Exit
A BT section carrying both perpendicular ports at once: a LEFT entry and a RIGHT exit, each seated clear of the flow end it faces. An upward flow starts at the bottom, so the entry seats below the row rather than above it (#1494).
Mermaid source
%%metro title: BT Left-Entry / Right-Exit Perpendicular Ports%%metro style: dark%%metro row_align: top%%metro line: dna | DNA | #e63946%%metro line: rna | RNA | #2db572
%%metro grid: qc | 0,0%%metro grid: rna_prep | 0,1%%metro grid: align | 1,0%%metro grid: calling | 2,0
graph LR subgraph qc [Quality Control] %%metro direction: BT %%metro exit: right | dna fastqc[FastQC] trim[Trim Galore] fastqc -->|dna| trim end
subgraph rna_prep [RNA Prep] %%metro direction: BT %%metro exit: right | rna umi[UMI Extract] end
subgraph align [Alignment] %%metro direction: BT %%metro entry: left | dna,rna %%metro exit: right | dna,rna bwa[BWA-MEM2] star[STAR] sort[Sort BAM] bwa -->|dna| sort star -->|rna| sort end
subgraph calling [Downstream Analysis] %%metro direction: BT %%metro entry: left | dna,rna variant[GATK Variant Calling] quant[Salmon Quant] end
trim -->|dna| bwa umi -->|rna| star sort -->|dna| variant sort -->|rna| quantCLI command
nf-metro render examples/topologies/bt_perp_left_entry_right_exit.mmd -o bt_perp_left_entry_right_exit.svg